4 × 44 k whole human genome microarray slides  (Agilent technologies)

Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: Scatter plot of the gene differential expression in PC-2 cells induced by RPM. Notes: The Cy3 (RPM treated group) and Cy5 (untreated control) channel intensities from the two-color DNA microarray experiments were shown in the scatter plot. The variables appear in a linear relationship, and the linear correlation between them is 0.935. The red plots represented upregulated genes, and the green plots represented downregulated genes. Abbreviation: RPM, rapamycin.

Article Snippet: The samples were subjected to mRNA expression profiling on 4 × 44 K Agilent Whole Human Genome Microarray slides (catalog G4112F; Agilent Technologies Inc), using a single-color array method.

Techniques: Expressing, Microarray

Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: ( A ) RT-PCR analysis for the DNA-damage repair and transcription genes after the treatment with RPM; ( B ) Difference between the cDNA microarray and RT-PCR in DNA-damage repair and transcription genes. Notes: The genes examined here are: (1) DDB1(NM_001923); (2) RAD51 (NM_002876); (3) XRCC5 (NM_021141); (4) PCNA (NM_002592); and (5) ABCC4 (NM_005845). The values represent the mean ± SD of the data from three independent experiments. Abbreviations: RPM, rapamycin; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: The samples were subjected to mRNA expression profiling on 4 × 44 K Agilent Whole Human Genome Microarray slides (catalog G4112F; Agilent Technologies Inc), using a single-color array method.

Techniques: Reverse Transcription Polymerase Chain Reaction, Microarray, Standard Deviation

Journal: BMC Immunology

Article Title: Differential effects of Paclitaxel on dendritic cell function

doi: 10.1186/1471-2172-11-14

Figure Lengend Snippet: Microarray analysis of DC exposed to LPS 1 μg/ml or paclitaxel 100 μM for 2 hours.

Article Snippet: This was used as a probe on the Whole Human Genome Microarray (4 × 44 K) slide (Agilent).

Techniques: Microarray

Journal: BMC Complementary and Alternative Medicine

Article Title: Hot water extract of Agaricus blazei Murrill specifically inhibits growth and induces apoptosis in human pancreatic cancer cells

doi: 10.1186/s12906-018-2385-4

Figure Lengend Snippet: a Venn diagram analysis of global gene expression of pancreatic cancer cells treated with the hot water extract of Agaricus blazei Murrill (AbE). Genes with a statistically significant (greater than four-fold) change in expression were collected and classified. In the three cell lines, 394 genes were commonly altered. b Quantitative reverse transcription PCR was performed to validate the microarray results. The expression of genes associated with cell cycle progression or apoptosis was altered by the AbE treatment. The mean changes in gene expression relative to those in untreated cells, calculated by using GAPDH as the reference housekeeping gene, are displayed on a log2 scale. The difference in expression of each mRNA relative to that of non-treated cells was determined using a Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: For each hybridization, 1.65 μg of cRNAs was fragmented at 60 °C for 30 min using the Gene Expression Hybridization Kit (Agilent Technologies) and hybridized at 65 °C for 17 h to a 4 × 44 K Whole Human Genome Microarray slide (Agilent Technologies) in accordance with the manufacturer’s instructions.

Techniques: Expressing, Microarray

Journal: Journal of Dental Research

Article Title: Epithelial TRPV1 Signaling Accelerates Gingival Epithelial Cell Proliferation

doi: 10.1177/0022034514552826

Figure Lengend Snippet: Expression of candidate genes related to TRPV1-mediated cell proliferation. Epi 4 cells were stimulated with 1 µM capsaicin for 4 hr, and the gene expression profiles were analyzed by microarray. Among the up-regulated genes found to be related to cell proliferation by microarray analysis, statistical validation for fibroblast growth factor (FGF)-17 and neuregulin (NRG) 2 was discovered by independent experiments using quantitative polymerase chain-reaction. *p < .05; **p < .01 vs. unstimulated cells.

Article Snippet: Following labeling and purification, Cy3-labeled cRNAs were competitively hybridized onto an Agilent 4 × 44 K whole human genome oligo microarray slide (Agilent Technologies, Inc.), then scanned in an Agilent GeneArray Scanner (Agilent Technologies, Inc.).

Techniques: Expressing, Microarray, Real-time Polymerase Chain Reaction